The pH meter measures the voltage potential (mV) difference between the inside of the pH electrode and the outside sample (reference)

Glass pH electrodes develop a voltage across a permeable glass membrane.

The voltage is related to the difference in H+ activity inside and outside the glass.

pH

 

 

 

Electrode Selection

§   Select proper reference for application

§   Select proper junction for application

§   Select appropriate body and membrane profile

       Standard, semi-micro, micro, rugged bulb, spear tip, flat surface

§   Select appropriate body type

       Glass body, epoxy body

§   Select refillable or gel filled

 

 

Advantages of Thermo’s ROSS electrode

§   Advantages

       Variety of body styles

       Very stable reference

       Fast response

       Stable to 0.01 pH in 30 seconds over 50 o C temperature change

       Drift less than .002 pH units/day

       0.01 pH precision

       No Ag/Cl in reference system

§   Can measure samples containing Cysteine ( milk & milk related products )

§   Better for TRIS buffers

§   Ag + is toxic – Ross is preferred choice for biological samples & culture media

       Ross electrodes are all double junction – popular choice for non aqueous pH measurements such a titration.

 

  best ph electrode

 

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